ABSTRACT
Therapeutic efficacy of EGTA (ethylene glycol bis (2-aminoethyl ether) tetraacetic acid) against cerium intoxicated mice was studied. Administration of cerium showed significant decrease in haemoglobin percentage, RBC counts and blood glucose level with an increase in the activity of serum transaminases and WBC counts. Decrease in the activity of alkaline phosphatase and glycogen content was noted in liver and kidney after cerium exposure. Light and electron microscopical investigations showed that these changes were recouped considerably with the administration of EGTA suggesting its therapeutic efficacy against cerium toxicity.
Subject(s)
Alkaline Phosphatase/metabolism , Amidinotransferases/metabolism , Animals , Blood Glucose/metabolism , Cerium/administration & dosage , Chelating Agents/therapeutic use , Egtazic Acid/therapeutic use , Erythrocyte Count , Glycogen , Hemoglobins/metabolism , Kidney/drug effects , Leukocyte Count , Liver/drug effects , Male , MiceABSTRACT
Intermediary enzymes of streptomycin biosynthesis-arginine amidinotransferase and alkaline phosphatase were located at the early fermentation stages. Their relationship with streptomycin production by Streptomyces griseus strains GX-19 and MR-20 was determined and the use of this relationship was made for the screening of streptomycin producing mutant strains.